甘草渣中黄酮的提取及含量测定(附件)
摘 要甘草为豆科多年生草本性植物，作为最常用的、最重要的中药材之一的它被大家叫做“国老”，且它的入药部分是它的根和茎。作为补脾益气、祛痰止咳、清热解毒、调和众药的良——甘草，具有来源广、药物的质量易控制、毒副作用几乎为零等优点，因而它成了新药、保健品及绿色食品添加剂等发展的新方向，而它的主要有效成分有甘草多糖、黄酮类、三萜皂苷等。而作为一种宝贵的、可再次循环利用的资源—甘草渣，则被广泛的应用在食品邻域、药品领域和化妆品领域中去；甘草渣这一工业废料不仅合理循环利用了甘草资源，保护了生态环境，同时还使它的医疗价值和经济价值得到了提高。本文采用超声波辅助提取甘草渣中黄酮，利用单因素和正交试验相结合的方法来确定最佳的提取工艺，此提取工艺方法具有操作简单、能耗低、得率高、损失小、持续提高甘草渣的利用率等优点，本实验系统地考察了乙醇的浓度、超声的时间、超声的功率和料液比等因素对提取率的影响，并测得其含量。目 录
摘 要 I
Abstract II
第1章 文献综述 1
1.1引言 1
1.2甘草简介及其发展状况 2
1.3甘草渣的研究进展 3
1.4甘草渣化学成分研究与分类 3
1.4.1甘草甜素 4
1.4.2黄酮类化合物 4
1.4.3 甘草多糖 4
1.4.4氮基酸类 5
1.4.5甘草三菇类 5
1.4.6生物碱类 5
1.5甘草黄酮药理作用 5
1.5.1抗氧化作用 5
1.5.2抗病毒作用 6
1.5.3抗肿瘤作用 6
1.5.4美白作用 6
1.5.5甘草黄酮的抗炎作用 6
1.5.6抗菌作用 7
1.5.7其它药理作用 7
1.6提取方法 7
1.6.1超声提取法 7
1.6.2微波提取法 8
1.6.3超临界萃取法 8
1.6.4酶解提取法 8
1.7研究目的及研究内容 9
第2章 实验部分 10
2.1材料与试剂 10
2.2仪器设备
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菌作用 7
1.5.7其它药理作用 7
1.6提取方法 7
1.6.1超声提取法 7
1.6.2微波提取法 8
1.6.3超临界萃取法 8
1.6.4酶解提取法 8
1.7研究目的及研究内容 9
第2章 实验部分 10
2.1材料与试剂 10
2.2仪器设备 10
2.3实验用试剂的配制 10
2.4实验方法 11
2.4.1 原材料预处理 11
2.4.2甘草渣总黄酮含量的测定 11
2.4.3确定最佳检测波长 11
2.4.4标准曲线的绘制 11
2.4.5测定样品中甘草渣总黄酮的含量 12
2.5单因素试验 12
2.5.1超声时间 12
2.5.2 乙醇浓度 13
2.5.3超声功率 13
2.5.4 料液比 13
2.6正交试验 13
2.7结果与分析 14
2.7.1芦丁标准曲线 14
2.7.2单因素实验结果 15
2.7.3正交试验 17
2.7.4验证性试验 19
2.8结论 19
参考文献 21
致谢 23
Abstract
Licorice for nature plant adversityresistant perennial grass, as the most commonly used, one of the most important Chinese traditional medicine, it is everybody is called "the old", and it is part of its root and stem. As tonifying spleen yiqi, expectorant, cough, qingrejiedu, adjustable and medicine, licorice, and has a wide source, quality advantages of easy control, and little or no side effects, therefore it became a new drugs, health products and the new development direction of green food additive, etc, and its main effective components of glycyrrhiza polysaccharide, flavonoids and triterpenoid saponins, etc. As a kind of valuable resources, can be recycled again, liquorice residue, neighborhood has been widely used in food, medicines and cosmetics fields; Licorice the industrial waste slag not only reasonable recycling the licorice resources, protect the ecological environment, at the same time also make its medical value and economic value is also improved.
This thesis mainly is to use single factor and orthogonal test method to optimize the combination of ultrasonic assisted extraction technology of liquorice residue medium yellow ketone, and because of this extraction process has simple operation, low energy consumption, high yield, low loss, continue to improve the utilization rate of liquorice residue, etc, so this experiment we put the ethanol concentration, ultrasonic time, ultrasonic power and material liquid ratio as the main factors of investigation, with liquorice residue medium yellow ketone content as indexes, through the single factor experiment and orthogonal design to optimize the extr
摘 要 I
Abstract II
第1章 文献综述 1
1.1引言 1
1.2甘草简介及其发展状况 2
1.3甘草渣的研究进展 3
1.4甘草渣化学成分研究与分类 3
1.4.1甘草甜素 4
1.4.2黄酮类化合物 4
1.4.3 甘草多糖 4
1.4.4氮基酸类 5
1.4.5甘草三菇类 5
1.4.6生物碱类 5
1.5甘草黄酮药理作用 5
1.5.1抗氧化作用 5
1.5.2抗病毒作用 6
1.5.3抗肿瘤作用 6
1.5.4美白作用 6
1.5.5甘草黄酮的抗炎作用 6
1.5.6抗菌作用 7
1.5.7其它药理作用 7
1.6提取方法 7
1.6.1超声提取法 7
1.6.2微波提取法 8
1.6.3超临界萃取法 8
1.6.4酶解提取法 8
1.7研究目的及研究内容 9
第2章 实验部分 10
2.1材料与试剂 10
2.2仪器设备
*好棒文|www.hbsrm.com +Q: *351916072*
菌作用 7
1.5.7其它药理作用 7
1.6提取方法 7
1.6.1超声提取法 7
1.6.2微波提取法 8
1.6.3超临界萃取法 8
1.6.4酶解提取法 8
1.7研究目的及研究内容 9
第2章 实验部分 10
2.1材料与试剂 10
2.2仪器设备 10
2.3实验用试剂的配制 10
2.4实验方法 11
2.4.1 原材料预处理 11
2.4.2甘草渣总黄酮含量的测定 11
2.4.3确定最佳检测波长 11
2.4.4标准曲线的绘制 11
2.4.5测定样品中甘草渣总黄酮的含量 12
2.5单因素试验 12
2.5.1超声时间 12
2.5.2 乙醇浓度 13
2.5.3超声功率 13
2.5.4 料液比 13
2.6正交试验 13
2.7结果与分析 14
2.7.1芦丁标准曲线 14
2.7.2单因素实验结果 15
2.7.3正交试验 17
2.7.4验证性试验 19
2.8结论 19
参考文献 21
致谢 23
Abstract
Licorice for nature plant adversityresistant perennial grass, as the most commonly used, one of the most important Chinese traditional medicine, it is everybody is called "the old", and it is part of its root and stem. As tonifying spleen yiqi, expectorant, cough, qingrejiedu, adjustable and medicine, licorice, and has a wide source, quality advantages of easy control, and little or no side effects, therefore it became a new drugs, health products and the new development direction of green food additive, etc, and its main effective components of glycyrrhiza polysaccharide, flavonoids and triterpenoid saponins, etc. As a kind of valuable resources, can be recycled again, liquorice residue, neighborhood has been widely used in food, medicines and cosmetics fields; Licorice the industrial waste slag not only reasonable recycling the licorice resources, protect the ecological environment, at the same time also make its medical value and economic value is also improved.
This thesis mainly is to use single factor and orthogonal test method to optimize the combination of ultrasonic assisted extraction technology of liquorice residue medium yellow ketone, and because of this extraction process has simple operation, low energy consumption, high yield, low loss, continue to improve the utilization rate of liquorice residue, etc, so this experiment we put the ethanol concentration, ultrasonic time, ultrasonic power and material liquid ratio as the main factors of investigation, with liquorice residue medium yellow ketone content as indexes, through the single factor experiment and orthogonal design to optimize the extr
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