猪12号染色体基因位点与二花脸猪产仔数关联性的研究

目录
摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法2
1.1实验材料 2
1.2实验方法 3
1.2.1样品DNA稀释液的制备3
1.2.2目标位点引物的设计3
1.2.3目的片段PCR扩增条件的摸索3
1.2.4目的片段PCR扩增4
1.2.5利用凝胶电泳技术对扩增结果进行检测4
1.2.6对目的片段进行DNA测序4
1.2.7DNA测序结果与总产仔数关联分析4
2结果与分析 4
2.1测序结果5
2.2结果分析 5
3讨论 6
3.1扩大群体进一步验证该位点的显著性 6
3.2进一步验证RPS6KB1上其他位点与产仔数的关联 6
3.3影响猪产仔数性状的其他因素 6
3.3.1年龄因素 6
3.3.2营养因素 6
3.3.3管理因素 6
3.3.4疫病因素 6
3.4 3’UTR区域在猪生产中的作用6
4总结 7
致谢7
参考文献8
猪12号染色体基因位点与二花脸猪产仔数关联性的研究
关键字：二花脸猪；RPS6KB1基因；产仔数；关联性
Association study of pig litter size with the chromosome 12 loci of Erhualian pigs
Student majoring in Animal Science Fan dongyao
Tutor Li Pinghua
Abstract: In the most economic traits of pig, litter size is the most important trait, it could reflects the production level of the pig. Rai *好棒文|www.hbsrm.com +Q: ^351916072* 
sing the number of the pigs could directly improve the production efficiency of the pigs and bring considerable economic benefit to the modern pig farm. However, pig litter size trait is a complex polygenic trait, and the heritability is very low, it is difficult to use traditional breeding method to improve litter size, so take advantage of new molecular breeding notation to improve litter size is becoming a hot spot of research.In the previous study, the instructors laboratory found QTLs associated with litter size in Pig No. 12 chromosome. And the RPS6KB1 gene located in the range of 100kb upstream and downstream of chromosome 12 was listed as a functional candidate gene affecting the variation of litter size in Erhualian population. This research typing the candidate loci of RPS6KB1 in 304 purebred Erhualian pigs bu using the PCR amplification and DNA sequencing technology, and then do the correlation analysis between genotype and phenotype by using SAS software. Our test is an exploratory test, the results showed that the genotypes have no significant correlation with both total litter size and litter number born alive in Erhualian population, which may imply RPS6KB1 3 UTR region 12:37431651 loci could not control pig litter size expression directly, so the followup work need to study the promoter regions and 3 UTR region for further research.

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