ebp1调节鸡胚骨骼肌发育的研究

目录
摘要................................................................................................................................1
关键词............................................................................................................................1
Abstract..........................................................................................................................1
Key words......................................................................................................................1
引言................................................................................................................................1
1.材料与方法.................................................................................................................3
1.1 实验材料、试剂、仪器.........................................................................................3
1.1.1 实验材料............................................ *好棒文|www.hbsrm.com +Q: ￥351916072$ 
..................................................................3
1.1.2 实验试剂..............................................................................................................3
1.1.3 实验仪器..............................................................................................................3
1.2 实验方法.................................................................................................................3
1.2.1 组织样品的采集..................................................................................................3
1.2.2 RNA的提取与测定..............................................................................................3
1.2.3 cDNA的合成........................................................................................................4
1.2.4 引物的设计、目的片段的克隆...........................................................................4
1.2.5 Ebp1的免疫印迹实验..........................................................................................4
1.2.6 1.5%的琼脂糖凝胶电泳检测...............................................................................5
1.2.7 冰冻切片的制作和荧光染色..............................................................................6
1.2.8 数据统计分析......................................................................................................6
基因检测.....................................................................................................................6
Ebp1的免疫印迹实验图............................................................................................8

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