氯噻啉金增强免疫层析分析方法研究

目录
摘要................................................................................................................................1
关键词............................................................................................................................1
Abstract..........................................................................................................................1
Keywords.......................................................................................................................1
引言................................................................................................................................2
1材料与方法.................................................................................................................2
1.1材料..........................................................................................................................2
1.1.1主要试剂...................... *好棒文|www.hbsrm.com +Q: ￥351916072￥ 
.........................................................................................2
1.1.2主要仪器...............................................................................................................2
1.1.3缓冲溶液 .............................................................................................................3
1.2方法..........................................................................................................................3
1.2.1胶体金制备...........................................................................................................3
1.2.2金标抗体制备及纯化...........................................................................................3
1.2.3链霉亲和素标记的纳米金探针的制备...............................................................3
1.2.4生物试剂的固定...................................................................................................4
1.2.5免疫层析试纸条的制备及使用方法...................................................................4
1.2.6试纸条和工作条件的优化...................................................................................5
1.2.7敏感性评价...........................................................................................................5
1.2.8选择性评价...........................................................................................................5
1.2.9添加回收实验.......................................................................................................6
1.2.10真实样品分析.....................................................................................................6
2结果分析.....................................................................................................................6
2.1胶体金颗粒鉴定......................................................................................................6
2.2胶体金标记单克隆抗体..........................................................................................6

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