基于荧光碳点的玉米赤霉烯酮印迹传感体系的制备及初步评价(附件)【字数：10716】
1
目 录
ABSTRACT 1
KEY WORDS 1
引言.1
1 材料与方法 3
1．1 材料 3
1．1．1 实验试剂 3
1．1．2 实验主要仪器与耗材 3
1．1．3 主要溶液的配制 3
1．2 方法 3
1．2．1 碳点的合成 3
1．2．2 碳点的表征分析 4
1．2．2．1 动态光散射分析(dynamic light scattering, DLS) 4
1．2．2．2 傅里叶红外分析 4
1．2．3 碳点的光谱性质分析 4
1．2．3．1 碳点的紫外吸收光谱分析 4
1．2．3．2 碳点的荧光光谱分析 5
1．2．4 CDs MIP与CDs NIP的合成 5
1．2．5 模板分子的洗脱 5
1．2．6 CDs MIP与CDs NIP表征分析 5
1．2．7 CDs MIP与CDs NIP的吸附实验 6
1．2．7．1 测试条件的确定 6
1．2．7．2 吸附介质的选择 6
1．2．7．3 吸附动力学实验 6
1．2．7．4 静态吸附实验 6
1．2．7．5 特异竞争性实验 6
2 结果与分析 6
2．1 碳点的合成 6
2．2 碳点的表征分析 7
2．2．1 动态光散射分析(dynamic light scattering, DLS) 7
2．2．2 傅里叶红外光谱分析 7
2．3 碳点的光谱性质 8
2．3．1 荧光碳点的紫外吸收光谱 8
2．3．2 碳点的荧光强度分析 8
2．3 CDs MIP与CDs NIP的合成 9
2．4 模板分子的洗脱 10
2．5 CDs MIP与CDs NIP的表征分析 10
2．5．1 紫外吸收光谱 *好棒文|www.hbsrm.com +Q: *351916072*
分析 10
2．5．2 扫描电镜表征测定 10
2．6 CDs MIP与CDs NIP的吸附实验 11
2．6．1 测试条件的确定 11
2．6．2 吸附介质的确定 11
2．6．3 吸附动力学 11
2．6．4 静态吸附实验 11
2．6．5 特异竞争性实验 13
3 讨论 13
3．1 碳点的合成 13
3．2 荧光分子印迹物检测体系的构建 13
致谢 13
参考文献 13
基于荧光碳点的玉米赤霉烯酮印迹传感体系
的制备及初步评价
动物医学专业学生 孙昭宇
指导教师 宋素泉
摘要：霉菌毒素玉米赤霉烯酮（Zearalenone, ZEN）具有肝肾、遗传、免疫及细胞毒性，广泛存在于各种谷物及动物饲料中，并能通过食物链进入人体，严重危害人和动物的健康，因此开发新型检测材料及技术并应用于ZEN含量的测定在动物性食品安全检测工作中具有重要意义。基于此，本课题首先制备了可产生蓝光的荧光碳点（carbon dots，CDs）响应元件，并对碳点的荧光特性、理化性质进行了表征，然后以该响应元件为核心，通过非水解溶胶凝胶技术构建了ZEN特异的荧光分子印迹聚合物(CDsMIP)。本研究建立的新型荧光碳点分子印迹传感体系结合了CDs的灵敏性与MIP的高特异性，可以高效而特异的识别ZEA，并对其进行定量，检测灵敏度达100ng 。
Preparation of Carbon DotsMolecular Imprinting based Fluorescent Biosensing Systems for Zearalenone Determination and Its Preliminary Evaluation
Student majoring in Veterinary Medicine Zhaoyu Sun
Tutor Suquan Song
Abstract：Mycotoxin zearalenone(ZEN) causes reproductive problems, infertility in farm animals, and causes severe liver and kidney problems due to its estrogenic activities, and It also has cytotoxicity, genotoxicity, immunetoxicity and carcinogenicity, endangers human and animals health. ZEN comtaminates cereal crops such as corn, barley and wheat. It can enter the body through the food chain. Therefore, the development of new material and technology for ZEN in animal food safety determination is of great significance. First, we synthesized the highly blue luminescent CDs via bottomup carbonization. The CDs acted as antennas for single amplification and optical readout. All CDs were characterized with the methods of fluorescence spectrum, ultraviolet spectrum, infrared spectrum, dynamic light scattering and transmission electron microscopy (TEM). Then CDs MIP and CDs NIP were formed through a nonhydrolytic solgel process. A newfangled sensitive and selective fluorescent biosensing systems for determination of ZEN, which was based on carbon dotsmolecular imprinting polymer(CDs MIP), was prepared by an single stage method. The CDs MIP specifically bound to template molecule zearalenone. Under optimized conditions, the relative fluorescence intensity of CDs MIP decreased linearly with the concentration of ZEN with a detection limit of 100ng.
目 录
ABSTRACT 1
KEY WORDS 1
引言.1
1 材料与方法 3
1．1 材料 3
1．1．1 实验试剂 3
1．1．2 实验主要仪器与耗材 3
1．1．3 主要溶液的配制 3
1．2 方法 3
1．2．1 碳点的合成 3
1．2．2 碳点的表征分析 4
1．2．2．1 动态光散射分析(dynamic light scattering, DLS) 4
1．2．2．2 傅里叶红外分析 4
1．2．3 碳点的光谱性质分析 4
1．2．3．1 碳点的紫外吸收光谱分析 4
1．2．3．2 碳点的荧光光谱分析 5
1．2．4 CDs MIP与CDs NIP的合成 5
1．2．5 模板分子的洗脱 5
1．2．6 CDs MIP与CDs NIP表征分析 5
1．2．7 CDs MIP与CDs NIP的吸附实验 6
1．2．7．1 测试条件的确定 6
1．2．7．2 吸附介质的选择 6
1．2．7．3 吸附动力学实验 6
1．2．7．4 静态吸附实验 6
1．2．7．5 特异竞争性实验 6
2 结果与分析 6
2．1 碳点的合成 6
2．2 碳点的表征分析 7
2．2．1 动态光散射分析(dynamic light scattering, DLS) 7
2．2．2 傅里叶红外光谱分析 7
2．3 碳点的光谱性质 8
2．3．1 荧光碳点的紫外吸收光谱 8
2．3．2 碳点的荧光强度分析 8
2．3 CDs MIP与CDs NIP的合成 9
2．4 模板分子的洗脱 10
2．5 CDs MIP与CDs NIP的表征分析 10
2．5．1 紫外吸收光谱 *好棒文|www.hbsrm.com +Q: *351916072*
分析 10
2．5．2 扫描电镜表征测定 10
2．6 CDs MIP与CDs NIP的吸附实验 11
2．6．1 测试条件的确定 11
2．6．2 吸附介质的确定 11
2．6．3 吸附动力学 11
2．6．4 静态吸附实验 11
2．6．5 特异竞争性实验 13
3 讨论 13
3．1 碳点的合成 13
3．2 荧光分子印迹物检测体系的构建 13
致谢 13
参考文献 13
基于荧光碳点的玉米赤霉烯酮印迹传感体系
的制备及初步评价
动物医学专业学生 孙昭宇
指导教师 宋素泉
摘要：霉菌毒素玉米赤霉烯酮（Zearalenone, ZEN）具有肝肾、遗传、免疫及细胞毒性，广泛存在于各种谷物及动物饲料中，并能通过食物链进入人体，严重危害人和动物的健康，因此开发新型检测材料及技术并应用于ZEN含量的测定在动物性食品安全检测工作中具有重要意义。基于此，本课题首先制备了可产生蓝光的荧光碳点（carbon dots，CDs）响应元件，并对碳点的荧光特性、理化性质进行了表征，然后以该响应元件为核心，通过非水解溶胶凝胶技术构建了ZEN特异的荧光分子印迹聚合物(CDsMIP)。本研究建立的新型荧光碳点分子印迹传感体系结合了CDs的灵敏性与MIP的高特异性，可以高效而特异的识别ZEA，并对其进行定量，检测灵敏度达100ng 。
Preparation of Carbon DotsMolecular Imprinting based Fluorescent Biosensing Systems for Zearalenone Determination and Its Preliminary Evaluation
Student majoring in Veterinary Medicine Zhaoyu Sun
Tutor Suquan Song
Abstract：Mycotoxin zearalenone(ZEN) causes reproductive problems, infertility in farm animals, and causes severe liver and kidney problems due to its estrogenic activities, and It also has cytotoxicity, genotoxicity, immunetoxicity and carcinogenicity, endangers human and animals health. ZEN comtaminates cereal crops such as corn, barley and wheat. It can enter the body through the food chain. Therefore, the development of new material and technology for ZEN in animal food safety determination is of great significance. First, we synthesized the highly blue luminescent CDs via bottomup carbonization. The CDs acted as antennas for single amplification and optical readout. All CDs were characterized with the methods of fluorescence spectrum, ultraviolet spectrum, infrared spectrum, dynamic light scattering and transmission electron microscopy (TEM). Then CDs MIP and CDs NIP were formed through a nonhydrolytic solgel process. A newfangled sensitive and selective fluorescent biosensing systems for determination of ZEN, which was based on carbon dotsmolecular imprinting polymer(CDs MIP), was prepared by an single stage method. The CDs MIP specifically bound to template molecule zearalenone. Under optimized conditions, the relative fluorescence intensity of CDs MIP decreased linearly with the concentration of ZEN with a detection limit of 100ng.
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