戊二醛癸甲溴铵溶液实验室微生物杀灭效果试验戊二醛癸甲溴铵溶液实验室微生物杀灭效果试验(附件)【字数：7405】
1为探究戊二醛癸甲溴铵溶液的微生物杀灭效果，本文以鸡新城疫病毒（Losata株）、猪瘟兔化弱毒、猪细小病毒（NADL-2株）为消毒对象进行杀灭效果试验，同时选用安灭杀作对照。结果表明:戊二醛癸甲溴铵溶液杀灭鸡新城疫病毒的有效浓度为1:4000，该浓度的消毒剂杀灭鸡新城疫病毒的最快有效时间为60min；戊二醛癸甲溴铵溶在5min内杀灭猪瘟兔化弱毒的最小稀释度为1:8000；戊二醛癸甲溴铵溶液杀灭猪细小病毒的有效浓度为1:1000，该浓度的消毒剂杀灭猪细小病毒的最快有效时间为5min；戊二醛癸甲溴铵溶液对病毒表现出强大的杀灭作用，但是其杀灭率随消毒剂稀释倍数增大或作用时间缩短而降低。
目 录
Abstract 1
Key words 1
1 材料与方法 2
1.1 试验材料 2
1.1.1 供试产品 2
1.1.2 试验毒株 2
1.1.3 试剂及试验动物 2
1.1.4 主要仪器 2
1.2 内容及方法 2
1.2.1 戊二醛癸甲溴铵溶液对鸡新城疫病毒的杀灭效果试验 2
1.2.2 戊二醛癸甲溴铵溶液对猪瘟兔化弱毒的杀灭效果试验 3
1.2.3 戊二醛癸甲溴铵溶液对猪细小病毒的杀灭试验 4
2 结果与分析 4
2.1 戊二醛癸甲溴铵溶液对鸡新城疫病毒的杀灭效果试验 4
2.1.1 消毒剂对鸡胚的安全性试验 4
2.1.2 中和剂和中和产物对鸡新城疫病毒及鸡胚生长的影响 5
2.1.3 对鸡胚的定量杀灭结果 5
2.1.4 对鸡胚的定性杀灭结果 5
2.2 戊二醛癸甲溴铵溶液对猪瘟兔化弱毒的杀灭效果试验 6
2.3 戊二醛癸甲溴铵溶液对猪细小病毒的杀灭试验 6
2.3.1 戊二醛癸甲溴铵溶液被中和后对PK15细胞生长的影响 6
2.3.2 戊二醛癸甲溴铵溶液对猪细小病毒的杀灭率的测定 6
3 讨论 7
致谢 7
参考文献 8
戊二醛癸甲溴铵溶 *好棒文|www.hbsrm.com +Q: ^351916072#
液实验室微生物杀灭效果试验
动物医学专业学生 张进
指导教师 吕英军
Test on sterilizing effect in laboratory of Glutaral and Deciquam Solution
Student majoring in veterinary Zhang Jin
Tutor Lv Yingjun
Abstract：To investigate the sterilizing effect of Glutaral and Deciquam Solution on microbes , sterilizing effect test was carried out with Newcastle disease virus(Losata), Hog cholera lapinized virus, and Porcine parvovirus(NADL2) as disinfection object in this paper, while selecting Compound Glutaral Solution as a contral. The results revealed that: The effective concentration of Glutaral and Deciquam Solution to kill the Newcastle disease virus(Losata) is 1:4000, the fastest time under this concentration is 60min. The minimum dilution degrees of Glutaral and Deciquam Solution to kill the Hog cholera lapinized virus is 1:8000 in five minutes; The effective concentration of Glutaral and Deciquam Solution to kill the Porcine parvovirus(NADL2) is 1:1000, the fastest time under this concentration is 5min. Glutaral and Deciquam Solution showed a powerful killing effect on those virus, but the killing rate of Glutaral and Deciquam Solution decreases with the increase of dilution times or the shortening of the action time.
Key words: Glutaral and deciquam solution; Newcastle disease virus(Losata); Hog cholera lapinized virus; Porcine parvovirus(NADL2); Sterilizing effect
由于单一消毒剂的消毒效果往往不太理想，亟需研究复合型消毒剂进行协同作用[15]，增强消毒性能。戊二醛是一种具有杀菌谱广、副作用低、性质稳定等诸多优点的消毒剂，被誉为化学消毒剂发展史上的里程碑之一[6]，是国内医疗器械消毒剂的首选[7]。而癸甲溴铵协同戊二醛能顺利进入微生物内部，改变蛋白质的结构和降低酶的活性，增强消毒作用，同时减小戊二醛的气味和腐蚀性[8]。戊二醛癸甲溴铵溶液对大肠杆菌[9]、链球菌[10]等细菌的灭菌试验已有相关研究，但对病毒的杀灭效果试验鲜有涉及。本试验探讨戊二醛癸甲溴铵溶液对病毒的杀灭作用，扩大其杀灭微生物的范围，将有助于戊二
目 录
Abstract 1
Key words 1
1 材料与方法 2
1.1 试验材料 2
1.1.1 供试产品 2
1.1.2 试验毒株 2
1.1.3 试剂及试验动物 2
1.1.4 主要仪器 2
1.2 内容及方法 2
1.2.1 戊二醛癸甲溴铵溶液对鸡新城疫病毒的杀灭效果试验 2
1.2.2 戊二醛癸甲溴铵溶液对猪瘟兔化弱毒的杀灭效果试验 3
1.2.3 戊二醛癸甲溴铵溶液对猪细小病毒的杀灭试验 4
2 结果与分析 4
2.1 戊二醛癸甲溴铵溶液对鸡新城疫病毒的杀灭效果试验 4
2.1.1 消毒剂对鸡胚的安全性试验 4
2.1.2 中和剂和中和产物对鸡新城疫病毒及鸡胚生长的影响 5
2.1.3 对鸡胚的定量杀灭结果 5
2.1.4 对鸡胚的定性杀灭结果 5
2.2 戊二醛癸甲溴铵溶液对猪瘟兔化弱毒的杀灭效果试验 6
2.3 戊二醛癸甲溴铵溶液对猪细小病毒的杀灭试验 6
2.3.1 戊二醛癸甲溴铵溶液被中和后对PK15细胞生长的影响 6
2.3.2 戊二醛癸甲溴铵溶液对猪细小病毒的杀灭率的测定 6
3 讨论 7
致谢 7
参考文献 8
戊二醛癸甲溴铵溶 *好棒文|www.hbsrm.com +Q: ^351916072#
液实验室微生物杀灭效果试验
动物医学专业学生 张进
指导教师 吕英军
Test on sterilizing effect in laboratory of Glutaral and Deciquam Solution
Student majoring in veterinary Zhang Jin
Tutor Lv Yingjun
Abstract：To investigate the sterilizing effect of Glutaral and Deciquam Solution on microbes , sterilizing effect test was carried out with Newcastle disease virus(Losata), Hog cholera lapinized virus, and Porcine parvovirus(NADL2) as disinfection object in this paper, while selecting Compound Glutaral Solution as a contral. The results revealed that: The effective concentration of Glutaral and Deciquam Solution to kill the Newcastle disease virus(Losata) is 1:4000, the fastest time under this concentration is 60min. The minimum dilution degrees of Glutaral and Deciquam Solution to kill the Hog cholera lapinized virus is 1:8000 in five minutes; The effective concentration of Glutaral and Deciquam Solution to kill the Porcine parvovirus(NADL2) is 1:1000, the fastest time under this concentration is 5min. Glutaral and Deciquam Solution showed a powerful killing effect on those virus, but the killing rate of Glutaral and Deciquam Solution decreases with the increase of dilution times or the shortening of the action time.
Key words: Glutaral and deciquam solution; Newcastle disease virus(Losata); Hog cholera lapinized virus; Porcine parvovirus(NADL2); Sterilizing effect
由于单一消毒剂的消毒效果往往不太理想，亟需研究复合型消毒剂进行协同作用[15]，增强消毒性能。戊二醛是一种具有杀菌谱广、副作用低、性质稳定等诸多优点的消毒剂，被誉为化学消毒剂发展史上的里程碑之一[6]，是国内医疗器械消毒剂的首选[7]。而癸甲溴铵协同戊二醛能顺利进入微生物内部，改变蛋白质的结构和降低酶的活性，增强消毒作用，同时减小戊二醛的气味和腐蚀性[8]。戊二醛癸甲溴铵溶液对大肠杆菌[9]、链球菌[10]等细菌的灭菌试验已有相关研究，但对病毒的杀灭效果试验鲜有涉及。本试验探讨戊二醛癸甲溴铵溶液对病毒的杀灭作用，扩大其杀灭微生物的范围，将有助于戊二
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